Solution Information | help | |
Enzyme: | Glycogen synthase kinase-3 beta | |
inhibitor: | BDBM7263 | |
substrate: | GSK-3 Peptide Substrate | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.2 | |
Temp. Prep.: | 30 | |
Comments: | n/a | |
Solute 1: | DTT | |
Concentration: | 1 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | MgCl2 | |
Concentration: | 15 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | EGTA | |
Concentration: | 5 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | sodium vanadate | |
Concentration: | 1 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 5: | beta-glycerophosphate | |
Concentration: | 60 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 6: | p-nitrophenyl phosphate | |
Concentration: | 15 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | MOPS | |
Concentration: | 25 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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